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SRX25889161: RNA-Seq of soilHse
1 OXFORD_NANOPORE (PromethION) run: 79,975 spots, 119.5M bases, 101.8Mb downloads

Design: Extract high-quality DNA, and use Nanodrop to assess DNA purity, concentration, and integrity. Use agarose gel electrophoresis to analyze the fragment size distribution.Amplify the full-length 16S rRNA gene using specific primers, and precisely quantify the amplification products using Qubit. Analyze the fragment size distribution of the amplification products via agarose gel electrophoresis.Perform end repair and dA-tailing on the amplification products.Ligate sequencing adapters to the amplification products.Purify the library using magnetic beads.Load the library onto an R10.4 Flow Cell and perform sequencing using a PromethION P48 sequencer (Oxford Nanopore Technologies, Oxford, UK)
Submitted by: Hubei Minzu University
Study: The response of microbial communities and microbial interactions to long-term heavy metal exposure in selenium-rich carbonaceous rock soils.
show Abstracthide Abstract
This study aims to investigate the impact of heavy metals on microbial communities in the soils of carbonaceous rock deposits in selenium-rich areas. The structure and function of microbial communities in carbonaceous rock environments are regulated by various environmental factors, with heavy metals exerting a significant influence on these communities. We conducted an in-depth analysis of samples collected from carbonaceous rock deposits using high-throughput sequencing technology and various geochemical methods. The results indicated that heavy metals not only altered the composition of microbial communities but also significantly affected their ecological functions. Further analysis revealed the specific mechanisms by which different types of heavy metals influence microbial communities, providing new insights into microbial adaptation in heavy metal-polluted environments and offering a theoretical basis for bioremediation of heavy metal pollution.
Sample:
SAMN43401546 • SRS22487457 • All experiments • All runs
Organism: soil metagenome
Library:
Name: Hse-3
Instrument: PromethION
Strategy: RNA-Seq
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Runs: 1 run, 79,975 spots, 119.5M bases, 101.8Mb
Run# of Spots# of BasesSizePublished
SRR3046401379,975119.5M101.8Mb2024-08-29

ID:
34952043

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